mouse anti fgfr3c Search Results


mouse anti fgfr3c  (R&D Systems)
90
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Mouse Anti Fgfr3c, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-mapk (erk1 + erk2  (Thermo Fisher)
90
Thermo Fisher mouse anti-mapk (erk1 + erk2
Mouse Anti Mapk (Erk1 + Erk2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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real-time surface plasmon resonance biosensor biacore 4000  (Biacore)
90
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4g10 (antiphosphotyrosine)  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc 4g10 (antiphosphotyrosine)
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map kinase  (Thermo Fisher)
86
Thermo Fisher map kinase
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cleaved parp (asp214) antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc cleaved parp (asp214) antibody
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dm1a  (Cell Signaling Technology Inc)
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total anti-akt  (Cell Signaling Technology Inc)
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hrp anti-mouse  (Amersham Life Sciences Inc)
90
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cleaved parp asp214  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc cleaved parp asp214
Figure 5. Induction of apoptosis by NDGA, and comparison with Zilueton, another LOX inhibitor. A, HEK293-transfected cells were starved overnight before treatment with Zilueton or NDGA. Cells were collected and lysed in 1% NP40 Lysis Buffer. FGFR3-TDII was immunoprecipitated from 250 Ag of lysate, separated by 10% SDS-PAGE, and transferred to Immobilon-P membrane for immmunoblotting with antiphosphotyrosine (4G10; top) and FGFR3 antisera (bottom). Antibodies were detected by ECL. The membrane was stripped between each antibody incubation. B, apoptosis was assayed by PARP cleavage in HEK293 cells transfected with FGFR3-TDII or pcDNA3 (mock), treated for 24 h with a concentration range of NDGA in 10% FBS medium. Cells were collected and lysed in 1% NP40 Lysis Buffer. Thirty micrograms of lysate were separated by 15% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with <t>cleaved</t> <t>PARP</t> <t>(Asp214;</t> top), FGFR3 (middle), and h-tubulin antisera (bottom), followed by ECL. The membrane was stripped between each antibody incubation. KMS-18 (C) or KMS-11 (D) cells were treated for 24 h with 0, 5, 10, 20, or 30 Amol/L NDGA. Thirty micrograms of lysate was separated by 10% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted as in B.
Cleaved Parp Asp214, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti plcγ  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti plcγ
Figure 5. Induction of apoptosis by NDGA, and comparison with Zilueton, another LOX inhibitor. A, HEK293-transfected cells were starved overnight before treatment with Zilueton or NDGA. Cells were collected and lysed in 1% NP40 Lysis Buffer. FGFR3-TDII was immunoprecipitated from 250 Ag of lysate, separated by 10% SDS-PAGE, and transferred to Immobilon-P membrane for immmunoblotting with antiphosphotyrosine (4G10; top) and FGFR3 antisera (bottom). Antibodies were detected by ECL. The membrane was stripped between each antibody incubation. B, apoptosis was assayed by PARP cleavage in HEK293 cells transfected with FGFR3-TDII or pcDNA3 (mock), treated for 24 h with a concentration range of NDGA in 10% FBS medium. Cells were collected and lysed in 1% NP40 Lysis Buffer. Thirty micrograms of lysate were separated by 15% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with <t>cleaved</t> <t>PARP</t> <t>(Asp214;</t> top), FGFR3 (middle), and h-tubulin antisera (bottom), followed by ECL. The membrane was stripped between each antibody incubation. KMS-18 (C) or KMS-11 (D) cells were treated for 24 h with 0, 5, 10, 20, or 30 Amol/L NDGA. Thirty micrograms of lysate was separated by 10% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted as in B.
Anti Plcγ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p akt  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti p akt
Figure 5. Induction of apoptosis by NDGA, and comparison with Zilueton, another LOX inhibitor. A, HEK293-transfected cells were starved overnight before treatment with Zilueton or NDGA. Cells were collected and lysed in 1% NP40 Lysis Buffer. FGFR3-TDII was immunoprecipitated from 250 Ag of lysate, separated by 10% SDS-PAGE, and transferred to Immobilon-P membrane for immmunoblotting with antiphosphotyrosine (4G10; top) and FGFR3 antisera (bottom). Antibodies were detected by ECL. The membrane was stripped between each antibody incubation. B, apoptosis was assayed by PARP cleavage in HEK293 cells transfected with FGFR3-TDII or pcDNA3 (mock), treated for 24 h with a concentration range of NDGA in 10% FBS medium. Cells were collected and lysed in 1% NP40 Lysis Buffer. Thirty micrograms of lysate were separated by 15% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with <t>cleaved</t> <t>PARP</t> <t>(Asp214;</t> top), FGFR3 (middle), and h-tubulin antisera (bottom), followed by ECL. The membrane was stripped between each antibody incubation. KMS-18 (C) or KMS-11 (D) cells were treated for 24 h with 0, 5, 10, 20, or 30 Amol/L NDGA. Thirty micrograms of lysate was separated by 10% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted as in B.
Rabbit Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. Induction of apoptosis by NDGA, and comparison with Zilueton, another LOX inhibitor. A, HEK293-transfected cells were starved overnight before treatment with Zilueton or NDGA. Cells were collected and lysed in 1% NP40 Lysis Buffer. FGFR3-TDII was immunoprecipitated from 250 Ag of lysate, separated by 10% SDS-PAGE, and transferred to Immobilon-P membrane for immmunoblotting with antiphosphotyrosine (4G10; top) and FGFR3 antisera (bottom). Antibodies were detected by ECL. The membrane was stripped between each antibody incubation. B, apoptosis was assayed by PARP cleavage in HEK293 cells transfected with FGFR3-TDII or pcDNA3 (mock), treated for 24 h with a concentration range of NDGA in 10% FBS medium. Cells were collected and lysed in 1% NP40 Lysis Buffer. Thirty micrograms of lysate were separated by 15% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with cleaved PARP (Asp214; top), FGFR3 (middle), and h-tubulin antisera (bottom), followed by ECL. The membrane was stripped between each antibody incubation. KMS-18 (C) or KMS-11 (D) cells were treated for 24 h with 0, 5, 10, 20, or 30 Amol/L NDGA. Thirty micrograms of lysate was separated by 10% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted as in B.

Journal: Cancer Research

Article Title: Nordihydroguaiaretic Acid Inhibits an Activated Fibroblast Growth Factor Receptor 3 Mutant and Blocks Downstream Signaling in Multiple Myeloma Cells

doi: 10.1158/0008-5472.can-08-0575

Figure Lengend Snippet: Figure 5. Induction of apoptosis by NDGA, and comparison with Zilueton, another LOX inhibitor. A, HEK293-transfected cells were starved overnight before treatment with Zilueton or NDGA. Cells were collected and lysed in 1% NP40 Lysis Buffer. FGFR3-TDII was immunoprecipitated from 250 Ag of lysate, separated by 10% SDS-PAGE, and transferred to Immobilon-P membrane for immmunoblotting with antiphosphotyrosine (4G10; top) and FGFR3 antisera (bottom). Antibodies were detected by ECL. The membrane was stripped between each antibody incubation. B, apoptosis was assayed by PARP cleavage in HEK293 cells transfected with FGFR3-TDII or pcDNA3 (mock), treated for 24 h with a concentration range of NDGA in 10% FBS medium. Cells were collected and lysed in 1% NP40 Lysis Buffer. Thirty micrograms of lysate were separated by 15% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with cleaved PARP (Asp214; top), FGFR3 (middle), and h-tubulin antisera (bottom), followed by ECL. The membrane was stripped between each antibody incubation. KMS-18 (C) or KMS-11 (D) cells were treated for 24 h with 0, 5, 10, 20, or 30 Amol/L NDGA. Thirty micrograms of lysate was separated by 10% SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted as in B.

Article Snippet: Antibodies were obtained from the following sources: FGFR3 (C-15), STAT1 (E-23), STAT3 (C-20), h-tubulin (H-235) from Santa Cruz Biotechnology; 4G10 (antiphosphotyrosine) from Upstate Biotechnology; Phospho-STAT1 (Tyr701), Phospho-STAT3 (Tyr705; D3A7), Phospho-p44/42 MAPK (Thr202/Tyr204; E-10), cleaved PARP (Asp214) from Cell Signaling; MAPK (ERK1 + ERK2) from Zymed; and horseradish peroxidase (HRP) anti-mouse and HRP anti-rabbit from Amersham.

Techniques: Comparison, Transfection, Lysis, Immunoprecipitation, SDS Page, Membrane, Incubation, Concentration Assay